宁夏医科大学基础医学院病原生物学与医学免疫学系;国龙骨科医院骨科;咸阳职业技术学院;
目的 探讨类风湿关节炎（RA）患者膝关节滑液中的细胞外囊泡（EVs）对脐静脉内皮细胞血管生成的影响并初步探讨其机制。方法 收集20例RA患者膝关节滑液，以20例骨关节炎（OA）患者的滑液为对照。采用差速超速离心法纯化EVs，用透射电镜和纳米颗粒追踪观察分析EVs的形态和大小。通过Western blot检测EVs的CD9、CD63、细胞色素C (Cyt-c)，及所含血管内皮细胞生长因子(VEGF)、赖氨酰氧化酶(LOX)、基质金属蛋白酶2(MMP2)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)水平。用2种EVs干预人脐静脉内皮细胞(HUVEC)，分别以CCK8法、TranswellTM小室试验、细胞划痕实验和Matrigel胶血管生成试验检测HUVEC增殖、迁移和血管生成。用Western blot检测EVs干预对HUVEC中PI3K/AKT通路的影响。结果 RA滑液来源的EVs(RA-EVs)和OA滑液来源的EVs(OA-EVs)均呈杯口状，直径在30～400 nm之间，表达CD63和CD9，不表达Cyt-c。RA-EVs中所携带的VEGF、LOX、MMP2、TNF-α和TGF-β1较OA-EVs多。与OA-EVs干预组相比，RA-EVs显著促进HUVEC增殖、迁移和血管生成，上调PI3K/AKT的磷酸化，而PI3K抑制剂能够抑制EVs诱导的血管生成。结论RA关节滑液中的EVs携带较多的促血管生成和炎症相关的细胞因子和酶，可通过激活PI3K/AKT通路促进血管生成，参与RA病理进展。
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基本信息:
DOI：10.13431/j.cnki.immunol.j.20250010
中图分类号:R593.22
引用信息:
[1]王凯博,徐传皓,田彦斌等.类风湿关节炎滑液来源的细胞外囊泡促进HUVEC血管生成[J].免疫学杂志,2025,41(02):72-79.DOI:10.13431/j.cnki.immunol.j.20250010.
基金信息:
国家自然科学基金（32060178); 宁夏自然科学基金（2024AAC03257）